The total rate of ODs among workers of main shops in both enterprises who were actually exposed to harmful factors were extremely high: the rate for Apatite JSC was 25 times higher than in Russia and 15 times higher than in Murmansk Oblast, while the rate for Kolskaya MSC was about 30 and 20 times greater than in Russia and in Murmansk Oblast, respectively in the 2000s the difference reached 100–150 times. The total number of ODs and 5 specific categories (musculoskeletal, respiratory, nervous diseases, hearing loss and vibration disease were analysed. Data were retrieved from the Murmansk Oblast ODs database for the oblast and 2 enterprises – Apatite JSC and Kolskaya MSC – which contributed to more than half of the ODs in the oblast in 1980–2010. The general aim was to obtain an accurate estimate of ODs in Murmansk Oblast in 1980–2010 and to determine the rate of specific types of ODs among cohorts of workers who had been exposed to the hazardous factors causing the disease. Official statistics tend to underestimate the incidence of occupational disease (OD nationally and regionally in Russia. Occupational diseases in Murmansk Oblast: 1980–2010įull Text Available Background. Based on these results, we propose a tentative model for the architecture of the chicken egg-coat matrix that might be applicable to other vertebrate ones. Our results suggest that the ZP1 and ZP3 bind through their ZP-C domains to form the ZP1- ZP3 complexes and fibrils, which are assembled into bundles through interactions between the repeat domains of ZP1 to form the ZP1- ZP3 matrix, and that the ZPD molecules self-associate and bind to the ZP1- ZP3 matrix through its ZP-N and ZP-C domains to form the egg-coat matrix. We further confirmed interdomain interactions of the ZP glycoproteins by far-Western blot analyses of the egg-coat proteins and pull-down assays of ZP1 in the serum, using recombinant domains of ZP glycoproteins as probes. Here, we deduced the orientations and/or distributions of chicken ZP glycoproteins, ZP1, ZP3 and ZPD, in the egg-coat matrix by confocal immunofluorescent microscopy, and in the ZP1- ZP3 complexes generated in vitro by co-immunoprecipitation assays. It is suggested that the physiological functions of the egg coat are mediated and/or regulated coordinately by peptide and carbohydrate moieties of the ZP glycoproteins that are spatially arranged in the egg coat, whereas a comprehensive understanding of the architecture of vertebrate egg-coat matrix remains elusive. The egg coat plays important roles in fertilization, especially in species-specific interactions with sperm to induce the sperm acrosome reaction and to form the block to polyspermy. The vertebrate egg coat, including mammalian zona pellucida, is an oocyte-specific extracellular matrix comprising two to six zona pellucida ( ZP) glycoproteins. Okumura, Hiroki Sato, Takahiro Sakuma, Rio Fukushima, Hideaki Matsuda, Tsukasa Ujita, Minoru Identification of distinctive interdomain interactions among ZP-N, ZP-C and other domains of zona pellucida glycoproteins underlying association of chicken egg-coat matrix. Keywords: Dot blotting, ELISA, b ZP3, anti-b ZP3 Anti-b ZP3 synthetized by b ZP3 induced on rabbit detectable by immunohistochemistry methods on late primary oocytes, early secondary oocytes, growing secondary oocytes, and oocytes on de Graaf folicular phase. Highest titer showed on 31th day post immunization. Measurement with ELISA methods showed increased titer on OD405. Anti-b ZP3 increasing on 24th day and 31th day and still until 48th day. This research concludes spesific binding of anti-b ZP3 with increasing purple colour on dot blotting methods. Rabbit serum injected to rat for producing Rat Anti Rabbit Anti-b ZP3. Control female rabbit injected by Tris-Cl buffer diluted in Freund's adjuvant without b ZP3 antigen. Sub cutan induction of b ZP3 with Freund's adjuvant, CFA (Complete Freund's Adjuvant for initial immunization and following by IFA (Incomplete Freund's Adjuvant at the 14th day and 39th day. Purpose of this research is to detect spesific binding anti-b ZP3 to rabbit oocytes using dot blotting and ELISA method. Immunogenic sites of bovine zonna pellucidae 3 (b ZP3 could stimulated the presence of anti-b ZP3 which be located on rabbit ZP and inhibit sperm-egg interaction on fertilization process. SPECIFICITY OF ANTIBODY BOVINE ZONNA PELLUCIDAE 3 (ANTI-b ZP3 TO RABBIT ZP3 BASED ON b ZP3 AS CONTRACEPTIVE ANTIGENSĭirectory of Open Access Journals (Sweden)įull Text Available Zonna pellucidae can be develop as antigen potential candidates based on reversible immunocontraceptive vaccines.